作者：崔淑芳，郝光榮，湯 球，孫 偉，曹 平
【摘要】 目的 研究奈米珍珠粉對大鼠深Ⅱ°燒傷創面癒合的.影響並與微米珍珠粉在燒傷癒合中進行效比較。方法 奈米珍珠粉、微米珍珠粉(0.5g/只)分別與甘油按1∶1配製成混合液。以微米珍珠粉組與自然癒合組作對照，奈米珍珠粉組為實驗組。測定大鼠14%深Ⅱ°燙傷後7、15、21天的創面癒合率、創面組織學觀察以及面板羥脯氨酸的含量。結果 奈米珍珠粉組創面癒合率較微米珍珠粉組、自然癒合組高(P＜0.05或P＜0.01)；燒傷後3天各組面板羥脯氨酸含量均低於正常(P＜0.01)，奈米珍珠粉組減少程度小於其他組；傷後5天各組HP含量有所恢復，尤以奈米珍珠粉組顯著高於自然癒合組及微米珍珠粉組；10天后各組明顯升高，而奈米珍珠粉組HP含量的升高差異有非常顯著性(P＜0.01)。結論 奈米珍珠粉可以促進大鼠深Ⅱ°燒傷創面的癒合，其癒合作用明顯高於微米珍珠粉(P＜0.05)。
【關鍵詞】 奈米珍珠粉；微米珍珠粉；燒傷；羥脯氨酸
【Abstract】 Objective To study the efficiency comparison between nano and micro pearl powder for treatment of deep Ⅱ° skin scalds in rats.Methods Nano and micro pearl powder (0.5g each rat) are mixed with glycerol (1∶1) the micro pearl powder group and the normal healing group as the control，nano pearl powder were used as drug on the deep Ⅱ° skin scalds of rats in the experimental d healing rates was measured at 7，15，21 days post-injury；and the content of hydroxyproline (HP) were detected also.Results Wound healing rates of the nano pearl powder group were higher than that of the micro pearl powder group and the normal healing group (P＜0.05 or P＜0.01) the 3th day post-injury，the content of HP in every group were lower than that in the normal group (P＜0.01)，while the decrease in the nano pearl powder group was slight than those in other the 5th day post-injury，the content of OHP recovered to some extent，with a significantly higher elevation in the nano pearl powder group than those in other the 10th day post-injury，evident HP increase was found in every group，with a statistical significant higher elevation in the nano pearl powder group (P＜0.01).Conclusion Nano pearl powder could promote the wound healing of deep Ⅱ° skin scalds in rats significantly，it’s healing was more higher than that of micron pearl powder.
【Key words】 nano pearl powder；micro pearl powder；scald；hydroxyproline(HP)
This research is to study the wound healing efficacy of nano pearl powder in treatment of deep Ⅱ°skin scalds in rats and compare the function with those of micron pearl powder.
1 Material and methods
1.1 Pearl powder (Zhejiang Fenix Pharmaceutical Co.，Ltd)，nano and micro pearl powder (0.5g each rat ) are mixed with glycerol (1∶1)al hydroxyproline detection kit (Nanjing Jiancheng biotechnology Institute，Approved Number 20040631).
1.2 Experimental animals Female SD rats (n＝190，body weight 240～260g，Experimental Animal Center，Second Military Medical University) rats were fedin metabolic cages for 1 week.
Qualified certificate for experimental animal ［SCXK(Hu)2002-0006］；qualified certificate for animal facility ［(Hu)2002-0026］.
1.3 Establishment of deep Ⅱ° skin scalds model in rats The model was made based on the traditional method set up by Yang Taimei［1］ rats were denuded with BaS and scalded for 15s in 80℃ water the next day to get deep Ⅱ° skin scalds model in rats with a wound accounting for 14% of the body fig.1.
1.4 Methods Those rats were divided into 3 groups after the burn (n=60)：nano pearl powder group，micro pearl powder and normal healing nano pearl powder and micro pearl powder groups the wound were daubed with a mixture of nano，micro pearl powder and glycerol once a day，while the rats in the normal healing group were fed experiment lasted 21 days.
1.5 Observations
1.5.1 Wound healing circumstance The wound development healing percentage and healing circumstance were observed in the whole healing rates were calculated on day 7、15、21 after d healing rate=healing surface/original skin lesion×100%rding the wound healing as the index，to judge the healing time.
1.5.2 Dermal hydroxyproline(HP)measurement Tissue in the wound were taken from the 3 groups of rats on day 3，5，10，15 and 21 after scalding with normal rats skin as a dorsal skin were denuded and separated from the connective tissue followed by a drying at 80℃，50mg of which were measured by dermal HP reagent content was recorded by μg/mg.
1.6 Data statistics and analysis Inputting the data into the computer，to analyze the data with the Excel software，a significance dissimilarity of group data was showed P＜0.05 or P＜0.01，the average data was showed x±s.
2 Results
2.1 Comparison of the healing time and the wound development healing rate The skin wound in the nano pearl powder group is clear with no effusion and the micro pearl powder group，there is a rat with effusion on the skin the control group 2 cases of effusion and one infection were found.
The wound healing time of nano pearl powder has a significance dissimilarity more than that of micro pearl powder (P<0.01)，the wound development healing rate is more quickly.(P<0.01，See table 1 for details).
Tab.1 Comparison of wound healing time and development healing rate of the different times (x±s，%)
0Note：compared with control group：*P＜0.05，**P＜0.01；compared with micro pearl powder group：△P＜0.05，△△P＜0.01
2.2 Changement of the scalded skin tissue HP content in different phases The HP content in the normal control group is (78.05±7.79)μg/ day 3 after the scald，the HP content in every group of animals were lower than that in the normal group (P＜0.01)，while the decrease in the nano pearl powder group was slight than those in other day 5，the HP recovered to some extent，with a significantly higher elevation in the nano pearl powder group than those in other day 10，evident HP increase was found in every group，with a statistical